Review




Structured Review

Fisher Scientific superscript iii cellsdirect cdna synthesis kit
RT-PCR analysis revealed the endogenous expression of krt4, krtt1c19e, notch1a, notch 2, notch 3, and dlc in keratinocytes. These cells were FACS-sorted for EGFP+ cells from Tg(krt4:lyn-EGFP ) and for tdTomato+ cells from Tg(krtt1c19e:tdTomato ). Whole genome <t>cDNA</t> was used as a positive control, and reactions without a template served as negative controls. Figure 4—figure supplement 1—source data 1. Original gel images for RT-PCR analysis displayed in , with labels. Figure 4—figure supplement 1—source data 2. Original files for RT-PCR analysis displayed in , without labels.
Superscript Iii Cellsdirect Cdna Synthesis Kit, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/superscript+cdna+kit/capacity+cdna+high+kit+reverse+transcription/pmc12327944-57-5-12
Average 86 stars, based on 1 article reviews
superscript iii cellsdirect cdna synthesis kit - by Bioz Stars, 2026-09
86/100 stars

Images

1) Product Images from "Cytoneme-mediated intercellular signaling in keratinocytes is essential for epidermal remodeling in zebrafish"

Article Title: Cytoneme-mediated intercellular signaling in keratinocytes is essential for epidermal remodeling in zebrafish

Journal: eLife

doi: 10.7554/eLife.97400

RT-PCR analysis revealed the endogenous expression of krt4, krtt1c19e, notch1a, notch 2, notch 3, and dlc in keratinocytes. These cells were FACS-sorted for EGFP+ cells from Tg(krt4:lyn-EGFP ) and for tdTomato+ cells from Tg(krtt1c19e:tdTomato ). Whole genome cDNA was used as a positive control, and reactions without a template served as negative controls. Figure 4—figure supplement 1—source data 1. Original gel images for RT-PCR analysis displayed in , with labels. Figure 4—figure supplement 1—source data 2. Original files for RT-PCR analysis displayed in , without labels.
Figure Legend Snippet: RT-PCR analysis revealed the endogenous expression of krt4, krtt1c19e, notch1a, notch 2, notch 3, and dlc in keratinocytes. These cells were FACS-sorted for EGFP+ cells from Tg(krt4:lyn-EGFP ) and for tdTomato+ cells from Tg(krtt1c19e:tdTomato ). Whole genome cDNA was used as a positive control, and reactions without a template served as negative controls. Figure 4—figure supplement 1—source data 1. Original gel images for RT-PCR analysis displayed in , with labels. Figure 4—figure supplement 1—source data 2. Original files for RT-PCR analysis displayed in , without labels.

Techniques Used: Reverse Transcription Polymerase Chain Reaction, Expressing, Positive Control

Related Articles

Isolation:

Article Title: Membrane potential drives the exit from pluripotency and cell fate commitment via calcium and mTOR
Article Snippet: .. RNA collection and DNase treatment were performed using the RNAqueous®-Micro Total RNA Isolation Kit (AM1931) and cDNA was synthesized with the SuperScript Vilo cDNA Synthesis Kit (Fisher Scientific 11754-050). qPCR measurements were collected using SYBR Green reagent (LifeTech-4367659) on a Step OnePlus instrument (Applied Biosciences). ..

Article Title: Membrane potential drives the exit from pluripotency and cell fate commitment via calcium and mTOR.
Article Snippet: .. RNA collection and DNase treatment were performed using the RNAqueous®-Micro Total RNA Isolation Kit (AM1931) and cDNA was synthesized with the SuperScript Vilo cDNA Synthesis Kit (Fisher Scientific 11754-050). qPCR measurements were collected using SYBR Green reagent (LifeTech-4367659) on a Step OnePlus instrument (Applied Biosciences). ..

Synthesized:

Article Title: Membrane potential drives the exit from pluripotency and cell fate commitment via calcium and mTOR
Article Snippet: .. RNA collection and DNase treatment were performed using the RNAqueous®-Micro Total RNA Isolation Kit (AM1931) and cDNA was synthesized with the SuperScript Vilo cDNA Synthesis Kit (Fisher Scientific 11754-050). qPCR measurements were collected using SYBR Green reagent (LifeTech-4367659) on a Step OnePlus instrument (Applied Biosciences). ..

Article Title: Membrane potential drives the exit from pluripotency and cell fate commitment via calcium and mTOR.
Article Snippet: .. RNA collection and DNase treatment were performed using the RNAqueous®-Micro Total RNA Isolation Kit (AM1931) and cDNA was synthesized with the SuperScript Vilo cDNA Synthesis Kit (Fisher Scientific 11754-050). qPCR measurements were collected using SYBR Green reagent (LifeTech-4367659) on a Step OnePlus instrument (Applied Biosciences). ..

cDNA Synthesis:

Article Title: Membrane potential drives the exit from pluripotency and cell fate commitment via calcium and mTOR
Article Snippet: .. RNA collection and DNase treatment were performed using the RNAqueous®-Micro Total RNA Isolation Kit (AM1931) and cDNA was synthesized with the SuperScript Vilo cDNA Synthesis Kit (Fisher Scientific 11754-050). qPCR measurements were collected using SYBR Green reagent (LifeTech-4367659) on a Step OnePlus instrument (Applied Biosciences). ..

Article Title: Silver nanoparticles at sub-cytotoxic levels increase enteric pathogen invasion by compromising intestinal epithelial barrier integrity
Article Snippet: RNA quality and concentrations were checked using a BioTek Cytation 5 spectrophotometer (Agilent Technologies, Santa Clara, CA). .. Reverse transcription for cDNA synthesis from purified RNA was performed using Superscript IV VILO Kit (Fischer Scientific, Hanover Park, IL) following the manufacturer’s protocol. .. Expression profile of 84 genes involved in epithelial cell junctions and barrier integrity was performed using the RT2 ProfilerTM PCR Array: Human Cell Junction Pathway Finder (Qiagen, Germantown, MD, USA).

Article Title: Membrane potential drives the exit from pluripotency and cell fate commitment via calcium and mTOR.
Article Snippet: .. RNA collection and DNase treatment were performed using the RNAqueous®-Micro Total RNA Isolation Kit (AM1931) and cDNA was synthesized with the SuperScript Vilo cDNA Synthesis Kit (Fisher Scientific 11754-050). qPCR measurements were collected using SYBR Green reagent (LifeTech-4367659) on a Step OnePlus instrument (Applied Biosciences). ..

Article Title: Cytoneme-mediated intercellular signaling in keratinocytes is essential for epidermal remodeling in zebrafish
Article Snippet: Commercial assay or kit , Gibco Stem Pro Accutase Cell Dissociation Reagent , Thermo Fisher Scientific , A1110501 , . .. Commercial assay or kit , SuperScript III CellsDirect cDNA Synthesis Kit , Fisher Scientific , 18-080-200 , . .. Commercial assay or kit , PowerUP SYBR Green Master Mix , Thermo Fisher Scientific , A25742 , .

Article Title: One-Week Kava Dietary Supplementation Increases Both Urinary N - and O -Glucuronides of NNAL, a Lung Carcinogen Major Metabolite, among Smokers.
Article Snippet: 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone (commonly known as NNK) is one of the most prevalent and potent pulmonary carcinogens in tobacco products that increases the human lung cancer risk.. Kava has the potential to reduce NNK and tobacco smoke-induced lung cancer risk by enhancing urinary excretion of 4-(methylnitrosamino)-1-(3-pyridyl)-1butanol (NNAL, the major metabolite of NNK) and thus reducing NNKinduced DNA damage.. In this study, we quantified N-glucuronidated NNAL (NNAL-N-gluc), O-glucuronidated NNAL (NNAL-O-gluc), and free NNAL in the urine samples collected before and after 1-week kava dietary supplementation.

Real-time Polymerase Chain Reaction:

Article Title: Membrane potential drives the exit from pluripotency and cell fate commitment via calcium and mTOR
Article Snippet: .. RNA collection and DNase treatment were performed using the RNAqueous®-Micro Total RNA Isolation Kit (AM1931) and cDNA was synthesized with the SuperScript Vilo cDNA Synthesis Kit (Fisher Scientific 11754-050). qPCR measurements were collected using SYBR Green reagent (LifeTech-4367659) on a Step OnePlus instrument (Applied Biosciences). ..

Article Title: Membrane potential drives the exit from pluripotency and cell fate commitment via calcium and mTOR.
Article Snippet: .. RNA collection and DNase treatment were performed using the RNAqueous®-Micro Total RNA Isolation Kit (AM1931) and cDNA was synthesized with the SuperScript Vilo cDNA Synthesis Kit (Fisher Scientific 11754-050). qPCR measurements were collected using SYBR Green reagent (LifeTech-4367659) on a Step OnePlus instrument (Applied Biosciences). ..

SYBR Green Assay:

Article Title: Membrane potential drives the exit from pluripotency and cell fate commitment via calcium and mTOR
Article Snippet: .. RNA collection and DNase treatment were performed using the RNAqueous®-Micro Total RNA Isolation Kit (AM1931) and cDNA was synthesized with the SuperScript Vilo cDNA Synthesis Kit (Fisher Scientific 11754-050). qPCR measurements were collected using SYBR Green reagent (LifeTech-4367659) on a Step OnePlus instrument (Applied Biosciences). ..

Article Title: Membrane potential drives the exit from pluripotency and cell fate commitment via calcium and mTOR.
Article Snippet: .. RNA collection and DNase treatment were performed using the RNAqueous®-Micro Total RNA Isolation Kit (AM1931) and cDNA was synthesized with the SuperScript Vilo cDNA Synthesis Kit (Fisher Scientific 11754-050). qPCR measurements were collected using SYBR Green reagent (LifeTech-4367659) on a Step OnePlus instrument (Applied Biosciences). ..

Reverse Transcription:

Article Title: In Vitro Development of Human iPSC-Derived Functional Neuronal Networks on Laser-Fabricated 3D Scaffolds.
Article Snippet: Neural progenitor cells generated from human induced pluripotent stem cells (hiPSCs) are the forefront of ′′brain-on-chip′′ investigations.. Viable and functional hiPSC-derived neuronal networks are shaping powerful in vitro models for evaluating the normal and abnormal formation of cortical circuits, understanding the underlying disease mechanisms, and investigating the response to drugs.. They therefore represent a desirable instrument for both the scientific community and the pharmacological industry.

Article Title: Silver nanoparticles at sub-cytotoxic levels increase enteric pathogen invasion by compromising intestinal epithelial barrier integrity
Article Snippet: RNA quality and concentrations were checked using a BioTek Cytation 5 spectrophotometer (Agilent Technologies, Santa Clara, CA). .. Reverse transcription for cDNA synthesis from purified RNA was performed using Superscript IV VILO Kit (Fischer Scientific, Hanover Park, IL) following the manufacturer’s protocol. .. Expression profile of 84 genes involved in epithelial cell junctions and barrier integrity was performed using the RT2 ProfilerTM PCR Array: Human Cell Junction Pathway Finder (Qiagen, Germantown, MD, USA).

Purification:

Article Title: Silver nanoparticles at sub-cytotoxic levels increase enteric pathogen invasion by compromising intestinal epithelial barrier integrity
Article Snippet: RNA quality and concentrations were checked using a BioTek Cytation 5 spectrophotometer (Agilent Technologies, Santa Clara, CA). .. Reverse transcription for cDNA synthesis from purified RNA was performed using Superscript IV VILO Kit (Fischer Scientific, Hanover Park, IL) following the manufacturer’s protocol. .. Expression profile of 84 genes involved in epithelial cell junctions and barrier integrity was performed using the RT2 ProfilerTM PCR Array: Human Cell Junction Pathway Finder (Qiagen, Germantown, MD, USA).

other:

Article Title: Molecular anatomy of the alligator dorsal telencephalon.
Article Snippet: RNA was either stored at 2808C in RNase-free water (Sigma) or used immediately for cDNA synthesis [SuperScript III 1st strand cDNA kit (Fisher Scientific)] following manufacturer instructions. cDNA was diluted in RNase-free water and stored at 2208C.

Article Title: Insights into the functional role of grass carp IL-8 in head kidney leukocytes: pro-inflammatory effects and signalling mechanisms.
Article Snippet: Funding information applied basic research foundation of sichuan province, Grant/Award Number: 2021YJ0277 Abstract Interleukin-8 (IL-8) is a critical chemokine regulating immune cells' chemotaxis as well as their physiological or pathological activations.. In fish cells, recombinant IL-8 proteins induced transcriptions of pro-inflammatory cytokines.. Nonetheless, the exact mechanisms underlying the function of fish IL-8 as a pro-inflammatory cytokine are still unclear.



Similar Products

99
Thermo Fisher superscript cdna synthesis kit
Superscript Cdna Synthesis Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/superscript+cdna+kit/Taq+DNA+Polymerase+Kit/pmc12892918-202-15-19
Average 99 stars, based on 1 article reviews
superscript cdna synthesis kit - by Bioz Stars, 2026-09
99/100 stars
  Buy from Supplier

97
Quanta Biosciences superscript cdna synthesis kit
Superscript Cdna Synthesis Kit, supplied by Quanta Biosciences, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/superscript+cdna+kit/qScript+cDNA+Synthesis+Kit/bio_rxiv__2025__10__15__682356-329-1-5
Average 97 stars, based on 1 article reviews
superscript cdna synthesis kit - by Bioz Stars, 2026-09
97/100 stars
  Buy from Supplier

86
Sangon Biotech superscript ii cdna synthesis kit
Superscript Ii Cdna Synthesis Kit, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/superscript+cdna+kit/cdna+first+kit+mirna+strand+synthesis/pm42277600-52-21-26
Average 86 stars, based on 1 article reviews
superscript ii cdna synthesis kit - by Bioz Stars, 2026-09
86/100 stars
  Buy from Supplier

96
TaKaRa superscript cdna synthesis kit
Superscript Cdna Synthesis Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/superscript+cdna+kit/SMARTer+PCR+cDNA+Synthesis+Kit/pm40921884-94-9-13
Average 96 stars, based on 1 article reviews
superscript cdna synthesis kit - by Bioz Stars, 2026-09
96/100 stars
  Buy from Supplier

99
Bio-Rad superscript cdna synthesis kit
Superscript Cdna Synthesis Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/superscript+cdna+kit/iScript+cDNA+Synthesis+Kit/10__1016_slash_j__isci__2025__114385-339-30-34
Average 99 stars, based on 1 article reviews
superscript cdna synthesis kit - by Bioz Stars, 2026-09
99/100 stars
  Buy from Supplier

99
Thermo Fisher superscript vilo complementary dna synthesis kit
Superscript Vilo Complementary Dna Synthesis Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/superscript+cdna+kit/DNA/pmc12576131-144-8-14
Average 99 stars, based on 1 article reviews
superscript vilo complementary dna synthesis kit - by Bioz Stars, 2026-09
99/100 stars
  Buy from Supplier

86
Fisher Scientific superscript iii cellsdirect cdna synthesis kit
RT-PCR analysis revealed the endogenous expression of krt4, krtt1c19e, notch1a, notch 2, notch 3, and dlc in keratinocytes. These cells were FACS-sorted for EGFP+ cells from Tg(krt4:lyn-EGFP ) and for tdTomato+ cells from Tg(krtt1c19e:tdTomato ). Whole genome <t>cDNA</t> was used as a positive control, and reactions without a template served as negative controls. Figure 4—figure supplement 1—source data 1. Original gel images for RT-PCR analysis displayed in , with labels. Figure 4—figure supplement 1—source data 2. Original files for RT-PCR analysis displayed in , without labels.
Superscript Iii Cellsdirect Cdna Synthesis Kit, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/superscript+cdna+kit/capacity+cdna+high+kit+reverse+transcription/pmc12327944-57-5-12
Average 86 stars, based on 1 article reviews
superscript iii cellsdirect cdna synthesis kit - by Bioz Stars, 2026-09
86/100 stars
  Buy from Supplier

99
TaKaRa superscript iv reverse transcriptase cdna synthesis kit
RT-PCR analysis revealed the endogenous expression of krt4, krtt1c19e, notch1a, notch 2, notch 3, and dlc in keratinocytes. These cells were FACS-sorted for EGFP+ cells from Tg(krt4:lyn-EGFP ) and for tdTomato+ cells from Tg(krtt1c19e:tdTomato ). Whole genome <t>cDNA</t> was used as a positive control, and reactions without a template served as negative controls. Figure 4—figure supplement 1—source data 1. Original gel images for RT-PCR analysis displayed in , with labels. Figure 4—figure supplement 1—source data 2. Original files for RT-PCR analysis displayed in , without labels.
Superscript Iv Reverse Transcriptase Cdna Synthesis Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/superscript+cdna+kit/TB+Green+Premix+Ex+Taq/pmc12387645-241-21-35
Average 99 stars, based on 1 article reviews
superscript iv reverse transcriptase cdna synthesis kit - by Bioz Stars, 2026-09
99/100 stars
  Buy from Supplier

Image Search Results


RT-PCR analysis revealed the endogenous expression of krt4, krtt1c19e, notch1a, notch 2, notch 3, and dlc in keratinocytes. These cells were FACS-sorted for EGFP+ cells from Tg(krt4:lyn-EGFP ) and for tdTomato+ cells from Tg(krtt1c19e:tdTomato ). Whole genome cDNA was used as a positive control, and reactions without a template served as negative controls. Figure 4—figure supplement 1—source data 1. Original gel images for RT-PCR analysis displayed in , with labels. Figure 4—figure supplement 1—source data 2. Original files for RT-PCR analysis displayed in , without labels.

Journal: eLife

Article Title: Cytoneme-mediated intercellular signaling in keratinocytes is essential for epidermal remodeling in zebrafish

doi: 10.7554/eLife.97400

Figure Lengend Snippet: RT-PCR analysis revealed the endogenous expression of krt4, krtt1c19e, notch1a, notch 2, notch 3, and dlc in keratinocytes. These cells were FACS-sorted for EGFP+ cells from Tg(krt4:lyn-EGFP ) and for tdTomato+ cells from Tg(krtt1c19e:tdTomato ). Whole genome cDNA was used as a positive control, and reactions without a template served as negative controls. Figure 4—figure supplement 1—source data 1. Original gel images for RT-PCR analysis displayed in , with labels. Figure 4—figure supplement 1—source data 2. Original files for RT-PCR analysis displayed in , without labels.

Article Snippet: Commercial assay or kit , SuperScript III CellsDirect cDNA Synthesis Kit , Fisher Scientific , 18-080-200 , .

Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Positive Control